Table 1 The profile of NGS test panel. The list of targeted genes, enrichment method, and coverage of the next-generation sequencing panel that is used in this study

Test panel

Arrhythmia panel

Target enrichment method

Hybridization with oligonucleotide probes (GRM v1)

Massively parallel sequencing

MiSeq Dx (150 bp × 2; paired-end)

Reference genome

hg19

Bioinformatic pipeline

BI_GRM v1.1 (Alignment: BWA, Variant calling: GATK)

Coverage

Mean coverage of depth (X): 240.93 X

% of target bases ≥ 10X: 100%

Basic target regions (30 genes; 117,771 bp)

Extended target regions (30 genes)

ABCC9

HCN4

PKP2

ACTN2

JUP

PRKAG2

AKAP9

KCND3

RANGRF

ANKRD1

KCNA5

RBM20

ANK2

KCNE1

RYR2

BAG3

LDB3

SALL4

CACNA1C

KCNE2

SCN1B

DES

LMNA

SCN2B

CACNA2D1

KCNE3

SCN3B

DSC2

MYH6

TBX5

CACNB2

KCNH2

SCN4B

DSG2

MYH7

TGFB3

CALM1

KCHJ2

SCN5A

DSP

NKX2-5

TMEM43

CASQ2

KCNJ5

SNTA1

EMD

NPPA

TNNI3

CAV3

KCNJ8

TRN

GJA5

PDLIM3

TNNT2

GPD1L

KCNQ1

TRPM4

HADHA

PLN

TTN